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. 2011 Jul 8;6(7):e22001. doi: 10.1371/journal.pone.0022001

Figure 2. Expression analysis of BAC dual-reporter deletion constructs.

Figure 2

BHK-21 cells were transfected with the RP11-265B8::Ex5a-EK-DsAmp (Dual Reporter) construct and derivatives individually containing deletions of the identified conserved non-coding regions. EGFP and DsRed-Express expression were measured by flow cytometry 72 hours post transfection. The expression of the different deletion constructs was compared to the expression of the control RP11-265B8::Ex5a-EK-DsAmp (Dual Reporter) construct. Assays were performed in triplicate on at least three independent occasions. Error bars represent standard error of the mean. *p<0.05, ***p<0.001 in comparison to the unmodified control RP11-265B8::Ex5a-EK-DsAmp (Dual Reporter) construct.