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. 2011 Jul 8;6(7):e22001. doi: 10.1371/journal.pone.0022001

Figure 3. DNA fragments utilized in expression analyses.

Figure 3

Schematic representation of PCR-amplified fragments containing the FXN promoter and conserved regions cloned into the pGL3-Basic vector. The size of each fragment (bp) and the inclusion of particular conserved regions are shown. The location of each of the identified conserved non-coding regions, their distance (kb) from exon 1 of the FXN gene and their coordinates (UCSC Genome Browser, Human March 2006 assembly) are indicated. (Not to scale.)