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. 2011 Jul 8;6(7):e22001. doi: 10.1371/journal.pone.0022001

Figure 7. Regulatory effects of a putative Oct-1 binding site located within conserved region 1.

Figure 7

(A) Two mutant constructs were generated by site directed mutagenesis within a putative Oct-1 binding site within the identified 17 bp nucleotide sequence. MUT 1 contains one additional extra base in the middle of the sequence and MUT 2 contains two bases substitution at the start of the sequence. (B) MUT 1 and MUT 2 were generated in the pGL3-Basic::5023 construct. Luciferase expression of the mutant constructs was analyzed following co-transfection of BE(2)-M17 cells with pGL3-Basic derivatives and Oct-1 transcription factor-encoding plasmid pCMV-AC::Oct-1. The overall levels of expression were then compared to the pGL3-Basic::5577 vector, the expression value of which was set as 1. Assays were performed in triplicate on at least three independent occasions. Error bars represent standard error of the mean. *p<0.05; **p<0.01; ***p<0.001.