Figure 6.

In vitro transcription of pUP_DR2, pUP_DR2/hU6 and pUP_{DR2-Expression} in the presence of TBPwt or TBP-DR2 respectively. In vitro transcription was performed and the positions of the individual transcripts are indicated. Three transcription samples of each template (lanes 1–3, pUP_DR2; lanes 4–6, pUP_{DR2-Expression}; lanes 7–9, pUP_DR2/hU6) were analysed in a transcription system based on 10 µl phosphocellulose fraction B plus 20 µl phosphocellulose fraction C (lanes 1, 4 and 7) and were supplemented with either 20 ng TBP-DR2 (lanes 2, 5 and 8) or 20 ng TBPwt (lanes 3, 6 and 9).