Figure 5. Analysis of the kinetics of GAPDH aggregation registered in the regime of heating at the rate of 1°C/min (10 mM Na-phosphate buffer, pH 7.5, containing 0.1 M NaCl).

(A) The initial parts of the dependences of the light scattering intensity on temperature obtained at the following concentrations of GAPDH: (1) 0.1, (2) 0.75 and (3) 1.5 mg/ml. Points are the experimental data. The solid curves were calculated from Eq. (4). (B and C) The dependences of parameters T ₀ and K _agg calculated from Eq. (4) on the GAPDH concentration, respectively.