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. 2011 Jun 21;345(1):69–86. doi: 10.1007/s00441-011-1200-z

Fig. 3.

Fig. 3

Electrophysiological properties of Nestin-GFP expressing cells in adult amygdala. a Morphology and current pattern of Nestin-GFP-expressing cells in the amygdala from adult Nestin-promoter-GFP transgenic mice. I Bright-field image of an amygdala slice showing the position of the patch pipette during recording. II Fluorescence image of GFP-expressing cells. III After recording, the cell (arrow) was dialyzed with Alexa Fluoro 594 via the pipette solution. IV Merged image verifying that GFP and Alexa Fluoro 594 labels correspond. Bar 120 μm (I), 15 μm (II–IV). V, left Membrane currents were evoked in the shown cell by applying 50-ms voltage steps ranging from –140 mV to +20 mV from a holding potential of –70 mV (for the recording protocol, see inset). Note the transient inward current. V, right Voltage curve obtained from data shown left; current amplitudes at the beginning (black circles) and at the end (open circles) of the voltage pulse were plotted against the holding potential. VI, left Membrane currents from the shown cell were evoked by 50-ms voltage steps ranging from –210 mV to +80 mV from a holding potential of –70 mV (for the recording protocol, see inset). VI, right Outwardly rectifying current to voltage curve was obtained from data shown left; current amplitudes at the beginning (black circles) and at the end (open circles) of the voltage pulse were plotted against the holding potential. VII As described in VI, another GFP-positive cell was characterized by an outwardly rectifying and inwardly rectifying current voltage curve. b Neuronal properties were observed in a few Nestin-GFP-expressing cells in the amygdala. I-III Fluorescence images of a GFP-expressing cell in the amygdala; this cell displays the sodium currents shown in V; (I) GFP, (II) Alexa Fluoro 594, (III) merged. Bar 25 μm. IV In the current clamp mode, action potentials could be recorded after the depolarizing current injection (note the depolarizing current-dependent frequency). V, VI Spontaneous activity was recorded under control conditions (V) or in the presence of bicuculline (20 μM, VI)