Fig. 2.

Functional IK_Ca channels are present in excised patches from EA.hy926 cells. a Representative single-channel recording out of seven independent experiments in inside-out patch exposed to 1 μM bath Ca²⁺ showing two types (large and intermediate conductance) of Ca²⁺-activated K⁺ single-channel activity. b Representative single-channel recording from four independent experiments in inside-out patch in the presence of bath Ca²⁺ as indicated. The patch was held at a holding potential of −80 mV. Pipette solution contained iberiotoxin (400 nM) and apamin (100 nM). Single-channel activity is denoted as downward deflections. Bars from the right indicate closed state of the channel