Fig. 6.
Effect of LPI on the kinetic properties of IKCa channels. a Representative single-channel recording in inside-out patch containing one active channel exposed to 1 μM Ca2+ prior (Control) and during the exposure to 1 μM LPI at the cytosolic side of the patch at a holding potential of −80 mV. Channel openings are shown as downward deflections. b The mean open time of the IKCa prior (Control) and after addition of 3 μM LPI (n = 9). c The mean closed time (frequency of openings) of the IKCa prior (Control) and after addition of LPI (n = 9). *P < 0.05 vs. control. d Open time histograms in the absence (Control) and presence of 1 μM LPI. In both cases, the open time was fitted to two exponentials (τo1 and τo2). LPI did not affect τo1 and slightly increased τo2. e Closed time histograms in absence (Control) and presence of 1 μM LPI. In both cases, the closed time was fitted to three exponentials (τc1, τc2, and τo2). LPI did not affect τc1, slightly decreased τc2, and strongly decreased τc3