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. Author manuscript; available in PMC: 2011 Jul 9.
Published in final edited form as: Oncogene. 2010 Mar 15;29(22):3217–3227. doi: 10.1038/onc.2010.68

Figure 4. Initial cell attachment is dependent on McTN formation as affected by tau.

Figure 4

(A) Over 1h, MCF7 cells stably overexpressing GFP-tau (MCF-7tau1 and MCF-7tau2) attach more efficiently (200% and 180%, respectively) compared to MCF-7 controls. Data (n=4) represents the mean percentage of attachment ± s.d. *P<0.01. (B) Over 1h, siTau1 and siTau2 attach less efficiently (67% and 46%, respectively) compared to ZR-75-1 controls. Data (n=3) is represented as the mean percentage of attachment ± s.d. *P<0.01. (C) Glowscale of luciferase-expressing cells injected into the tail vein of nude mice demonstrate equal initial signal retention in regions of interest (ROI) emcompassing the lungs. Over a period of 8d post-injection, MCF-7tau1-luc cells are more retained than control MCF-7GFP-luc cells. (D). On average over all injections, 61.8% and 44.4% of MCF-7tau1-luc cells (n=6) were retained in lung capillaries 1d and 8d post-injection. In comparison, 15.9% and 12.9% of control MCF-7GFP-luc cells (n=5) were retained at the same time points. This difference was significantly different for both 1d (*P<0.02) and 8d (**P<0.05) post-injection by a non-parametric t-test.