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. 2011 Mar;79(6):1670–1683. doi: 10.1111/j.1365-2958.2011.07553.x

Fig. 2.

Fig. 2

Site-specific integration and excision of 89K in the chromosome of S. suis 05ZYH33.

A. Schematic representation of the site-specific integration and excision of 89K. The left and right junctions (attL and attR), which are shown as red rectangles, are formed by recombination between the chromosomal attB site and the 89K attP site. The flanking genes are shown by solid arrows indicating the direction of transcription. The location and orientation of primers used for detection of integrated and excised 89K are indicated by thin arrows.

B. Detection of a circular extrachromosomal form of 89K and the empty chromosomal attB site following precise excision, by PCR analysis using primer pairs which are presented upon the lanes, with 05ZYH33 genomic DNA as the template.

C. Printout of the sequencing chromatogram of PCR products amplified with primer pair P2/P3, showing the attP site (boxed) formed by the joining of the two ends of 89K.

D. Printout of the sequencing chromatogram of PCR products amplified with primer pair P1/P4, showing the empty chromosomal attB site (boxed) upon 89K excision.