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. Author manuscript; available in PMC: 2011 Jul 10.
Published in final edited form as: Eur J Neurosci. 2008 Nov 21;28(12):2459–2473. doi: 10.1111/j.1460-9568.2008.06527.x

Figure 2.

Figure 2

α-synuclein knockdown protects differentiated human neurons from MPP+ toxicity. Differentiated SH-SY5Y and MESC2.10 cells were transfected with SNCA1 (knockdown) or a scrambled version of SNCA1 (scram control) and differentiated as normal. At the end of differentiation, cells were exposed to different concentrations of MPP+ for 16 hours and then live cells were stained green using calcein AM and dead cells red using ethidium homodimer-1. Representative photographs are shown of differentiated (A) SH-SY5Y and (B) MESC2.10 cells exposed to normal media or 1mM MPP+ with or without α-synuclein knockdown. 20x objective. (C) Viability was determined by counting live cells in six random 20x fields of view for each condition. Results are from 3 separate experiments performed in triplicate (± SEM). Survival of differentiated SH-SY5Y cells exposed to MPP+ was significantly improved by α-synuclein knockdown (two-way ANOVA, siRNA effect, p < 0.001). (D) MESC2.10 cells were also significantly protected by α-synuclein knockdown from MPP+ exposure (two-way ANOVA, siRNA effect, p < 0.001).