Table 2.
Effects of metal ions, chemicals, and detergents on PF2001Δ60 lipase activity. The enzyme (0.5 μg) was incubated at 70°C in 50 mM sodium phosphate buffer (pH 7.0) in the presence of the tested agent using MUF-Hep as substrate. After 10 min, the mixture was assayed for residual activity using the standard lipase assay. The activity of the control was considered 100%.
| Chemical | Concentration (mM) | Relative activity (%) |
|---|---|---|
| KCl | 10.0 | 107.5 |
| MgCl2 | 10.0 | 77.9 |
| CaCl2 | 10.0 | 54.8 |
| SDS | 1.0 | 37.0 |
| DTT | 1.0 | 94.6 |
| Triton X-100 | 6.4 | 47.0 |
| EDTA | 1.0 | 97.8 |
| 10.0 | 195.7 | |
| EGTA | 1.0 | 103.4 |
| 10.0 | 169.0 | |
| PMSF | 1.0 | 0.0 |