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. 2011 Jun 29;2011:712892. doi: 10.1155/2011/712892

Figure 3.

Figure 3

Dispo85E stimulates osteoblastogenesis and inhibits adipogenesis in the mesenchymal stem cell line C3H10T1/2. C3H10T1/2 cells were grown to confluence in a standard medium. One day after confluence, the medium was replaced with a permissive osteogenic/adipogenic medium containing Dispo85E at the indicated concentrations. At PID 12, the mRNA level of IGF-1 (a) and OCN (b) was analyzed by real-time PCR (n = 3). (c) ALP activity and (d) the effect of Dispo85E on cell viability were measured (n = 4). (e) Cells were induced with adipogenic medium and treated with drugs. At PID 12, the cells were stained with oil red O to assess lipid accumulation (magnification, 200-fold). (f) After 16 days of culture in adipogenic medium, cells were harvested, fixed, and stained with Nile red solution. Percentage of Nile-red-stained cells in the total population of each sample were quantified with FACScan flow cytometry (n = 3). **Indicates significant difference from control group at P < .01; *Indicates significance at P < .05.