Figure 3.

mRNA titration and balance. (A–C) Probe fluorescent signals, signal-to-background and dynamic range as a function of input mRNA mass. Duplicate labeling reactions containing equal amounts of placenta mRNA in both the Cy3 and Cy5 channels were labeled and hybridized to UniGEM V2 arrays. Each data point is an average from the two hybridizations. Probe fluorescence signal was converted to moles product using a standard curve. Range minimum values remained between 100 and 200 U for all hybridizations. (D and E) An aliquot of 50 or 400 ng placenta mRNA was labeled with Cy3 and hybridized to either 400 or 50 ng mRNA labeled with Cy5, respectively, in duplicate. Only one of the two hybridizations is shown. The axes are in arbitrary fluorescent units.