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. Author manuscript; available in PMC: 2011 Jul 10.
Published in final edited form as: Nat Cell Biol. 2009 Jan 18;11(2):172–182. doi: 10.1038/ncb1831

Figure 4.

Figure 4

CHD8 recruits histone H1 to the promoters of p53 target genes. (a) HEK293T cells expressing 3 × Flag–histone H1c were subjected to immunoprecipitation with anti-Flag and immunoblot analysis with anti-CHD8 antibodies. (b) Immunoprecipitation of HEK293T lysates with anti-CHD8 and immunoblot analysis with anti-histone H1 antibodies. (c) In vitro binding assay for recombinant CHD8S and histone H1c. (d) U2OS cells overexpressing CHD8S were treated with the genotoxic agents etoposide (ETOP, 20 µM) and doxorubicin (DXR, 0.5 µM) and subjected to ChIP with an anti-histone H1 antibody. (e) ChIP was performed for the BAX promoter (n.d., not detected). (f) U2OS cells infected with a retroviral vector for CHD8S+L-1 shRNA were subjected to ChIP. (g) U2OS cells overexpressing CHD8S were infected with a retroviral vector for p53 shRNA, incubated with ETOP and then subjected to ChIP. Data are mean ± s.d., n = 3 (d–g).