Table 1. Sequence of typical STM primers and the tags from which they were derived.

Tag no.	Tag sequence (5′→3′)	STM primer sequence	Tm (°C)a	Cycle no.b
9	GTGCAGAGACCATTGCGTTCAC	GCAGAGACCATTGCGTTCAC	58.7	30
43	GTGCAGCAGCGAACAGGTGGCG	CTGCAGCAGCGAACAGGTG	60.6	20
46	GTGCAGCAGGGCTAAACTCCAG	CCAGCAGGGCTAAACTCCAG	60.0	30
62	GTGCAGCGAGCAGGTGGCGTAG	CTGCAGCGAGCAGGTGGCGTAG	69.8	20
67	GTGCAGCGGCAAGAGGAGCAGG	CTGCAGCGGCAAGAGGAGCAG	67.8	20
123	GTGCAGACATCATGCGTGACTG	CTGCAGACATCATGCGTGACTG	62.1	30
135	GTGCAGATCGAGGTTTGCGTCC	CTGCAGATCGAGGTTTGCGTC	62.9	30
189	GTGCAGCCTTGTCTTTACTTGG	CTGCAGCCTTGTCTTTACTTG	55.4	35
210	GTGCAGCTCCACCACATCTCCG	CTGCAGCTCCACCACATCTCC	62.5	35
297	GTGCAGCAAAAGACTTCAGTGC	CTGCAGCAAAAGACTTCAGTGC	60.2	30
298	GTGCAGAGACCATTGCGTTCAC	CTGCAGAGACCATTGCGTTCAC	62.8	30
313	GTGCAGATCGTGATCCATGTTC	CTGCAGATCGTGATCCATGTTC	60.1	35
639	GTGCAGATTTTTCCAACTTCAG	CTGCAGATTTTTCCAACTTCAG	57.0	35
764	GTGCAGCAACACACGGGAAGAT	CTGCAGCAACACACGGGAAGA	64.1	25
773	GTGCAGCTGAGGACACTCTTCA	CTGCAGCTGAGGACACTCTTCA	60.1	30
804	GTGCAGCTGAGCTGGCGCTTTC	CTGCAGCTGAGCTGGCGCTTTC	69.2	35

^aMelting temperature of the STM primer, as determined by NetPrimer.

^bNumber of PCR cycles performed with final annealing at 57°C to amplify target SSR loci from genomic DNA.