Table 3.

Immunohistochemical and western blot analyses of PrP^Sc in tissue samples obtained from cattle intracerebrally challenged with H-type BSE

Tissue	Case code
	7749		9458		0728
	IHC	WB	IHC	WB	IHC	WB
Central nervous system
Cerebral cortex	+	+	+	+	+	+
Obex	+	+	+	+	+	+
Cerebrum	+	+	+	+	+	+
Spinal cord	+	+	+	+	+	+
Peripheral nervous system
Cauda equina	+	+	+	+	+	+
Dorsal root ganglia	+	+	+	+	+	+
Trigeminal ganglia	+	+	+	+	+	+
Cranial cervical ganglia	-	+	-	+	-	+
Stellate ganglia	-	+	-	+	-	+
Sympathetic trunk	-	+	-	+	-	+
Celiac-mesenteric ganglion complex	-	+	-	+	-	+
Vagus nerve	-	+	-	+	-	+
Facial nerve	-	+	-	+	-	-
Hypoglossal nerve	-	+	-	+	-	-
Phrenic nerve	-	+	-	+	-	+
Accessory nerve	-	-	-	+	-	+
Suprascapular nerve	-	+	-	+	-	+
Brachial plexus	-	+	-	+	-	+
Median nerve	-	+	-	+	-	+
Radial nerve	-	+	-	+	-	+
Sciatic nerve	-	+	-	+	-	+
Tibial nerve	-	+	-	+	-	+
Optic nerve	+	+	+	+	+	+
Retina	+	+	+	+	+	+
Pituitary gland	+	+	+	+	+	+
Adrenal gland	-	+	-	+	-	+
Lymphoid tissues
Spleen	-	-	-	-	-	-
Tonsils (palatine, pharyngeal, lingual)	-	-	-	-	-	-
Thymus	-	-	-	-	-	-
Parotid lymph node	-	-	-	-	-	-
Mandibular lymph node	-	-	-	-	-	-
Lateral retropharyngeal lymph node	-	-	-	-	-	-
Superficial cervical lymph node	-	-	-	-	-	-
Brachiocephalic lymph node	-	-	-	-	-	-
Axillary lymph node	-	-	-	-	-	-
Superficial inguinal lymph node	-	-	-	-	-	-
Subiliac lymph node	-	-	-	-	-	-
Popliteal lymph node	-	-	-	-	-	-
Hepatic lymph node	-	-	-	-	-	-
Internal iliac lymph node	-	-	-	-	-	-
External iliac lymph node	-	-	-	-	-	-
Mesenteric lymph node	-	-	-	-	-	-

IHC, immunohistochemistry; WB, western blotting; +, positive for PrP^Sc; -, negative for PrP^Sc