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. 2011 Jul 8;6(7):e21911. doi: 10.1371/journal.pone.0021911

Figure 2. CCN2 is required for the TGF-β induced activation of Smad1 and Erk1/2 pathways.

Figure 2

(A) Foreskin fibroblasts were stimulated with TGF-β for the indicated time periods and analyzed for Smad1, Smad3, Smad2 and Erk phosphorylation kinetics. (B,C) Fibroblasts were transduced with AdenoCCN2 siRNA or the control scrambled virus for 72 h followed by stimulation with TGF-β for the indicated time points. Phosphorylation status and the total protein levels of Smad1, Erk1/2, and Smad3 were determined by western blot.