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. 2011 Jul 8;6(7):e21921. doi: 10.1371/journal.pone.0021921

Figure 4. Structural analysis of M. sedula CdvB and CdvC homo and hetero-polymers.

Figure 4

(A) SEC elution profile (Superose 6) of CdvB and CdvC mixture (20 µM each). Aliquots of the column fractions were separated on 12% SDS-PAGE showing that the 7 ml void volume peak contained both proteins while the excess of CdvC was found in the 12 ml peak. (B) Negative staining EM image of the CdvB-CdvC polymers. (C) Negative staining EM image of the CdvB deletion mutant (CdvΔC). (D) Negative staining EM image of CdvC before incubation with CdvB. The insets in B and C correspond to higher magnification (×5).