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. 2011 Jul 8;6(7):e21941. doi: 10.1371/journal.pone.0021941

Figure 1. (A) Intrinsic transcript cleavage property of RNAP.

Figure 1

Stalled elongation complexes bearing the 20 mer transcript were generated with M. smegmatis (Ms), M. tuberculosis (Mtb) and E. coli (Ec) RNAP respectively. The complexes were incubated for a prolonged time (1–4 hrs) in transcription buffer (pH 7.5), followed by resolving the cleavage products on 20% urea-PAGE. (B) pH-induced transcript-cleavage activity of RNAP. The gels show cleaved RNA generated from the 20 mer ternary complexes formed by Ms, Mtb and Ec RNAP in buffers of pH 6.0 to 10.0.