|
M. smegmatis SM07sigA
|
(HygR, his-rpoC, pJAM2mysA) |
[46], [47]
|
|
M. smegmatis mc2 155 |
(A high efficiency transformation strain of M. smegmatis) |
[45]
|
|
M. tuberculosis H37Ra
|
(An attenuated strain of M. tuberculosis H37Ra) |
[48]
|
|
E. coli BL21 |
(hsdS gal (λcIts857 ind1 Sam7 nin5 lacUV5-T7 gene 1) |
[51]
|
|
E. coli TK1001 |
MC1061 zgj-203::Tn10
|
[39]
|
|
E. coli TK1021 |
MC1061 greA::kan, greB::cat, zgj-203::Tn10
|
[39]
|
| pMS002 |
Derivative of pBR322 containing Mtb greA gene |
[39]
|
| pMV261 |
E. coli-mycobacteria shuttle vector with a hsp60 promoter |
[49]
|
| pET20bgre
|
M. tuberculosis gre cloned between NdeI and HincII of pET20b |
This study |
| pET20brv3788
|
M. tuberculosis Rv3788 cloned between NdeI and HindIII of pET20b |
This study |
| pET20bmsgre
|
M. smegmatis gre cloned between NdeI and HindIII of pET20b |
This study |
| pET20bgre-his |
M. tuberculosis gre cloned in pET20bwith C-terminus his-tag |
This study |
| pET20bgre-his |
M. tuberculosis gre S127E cloned in pET20b with C-terminus his-tag |
This study |
| pET20bEcgreA-his |
E. coli greA cloned in pET20b with C-terminus his-tag |
This study |
| pMVgreAS
|
M. tuberculosis gre cloned in anti-sense orientation under hsp60 promoter |
This study |
| pMVgreOE
|
M. tuberculosis gre coading sequence cloned under hsp60 promoter |
This study |
| pTrc99gre
|
M. tuberculosis gre cloned under trc promoter |
This study |
| Gre D43N Mut |
5′ GAAGAAGGCAACCTGCGCGAGAAC 3′
|
This study |
| Gre E46R Mut |
5′ GAAGGCGACCTGCGCCGTAACGGCGGATACCAC 3′
|
This study |
| Gre S127E Mut |
5′ TACTCGCCGAATGAACCGCTCGGTGGG 3′
|
This study |
| greBamH1For |
5′ ACGGATCCCGACCATATGACGGATACTCAAGTC 3′
|
This study |
| greBamH1Rev |
5′ ACGGATCCCGACCTGCTCGGAGATCTCGAACAG 3′
|
This study |
| greNdeIFor |
5′ CGACCATATGACGGATACTCAAGTC 3′
|
This study |
| greHindIIIRev |
5′ ATAAGCTTCGACCTGCTCGGAGATCTCGAACAG 3′
|
This study |
| rv3788NdeIFor |
5′ ATGCGACATATGAGCGAGAAAGTCGAGTC 3′
|
This study |
| rv3788HindIIIRev |
5′ ATAAGCTTTTCTGAGGGCAGCTTGACAG 3′
|
This study |
| MsgreNdeIFor |
5′ ATGCGACATATGACCGATACCCAGGTCACC 3′
|
This study |
| MsgreHindIIIRev |
5′ ATAAGCTTTCCGCCTTGATACGGCTCAGC 3′
|
This study |
