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Pretreatment (2 hours) with inhibitors of the p50 and p65 subunits of nuclear factor (NF)-κB decreased interleukin (IL)-1β-induced nuclear translocation of each subunit (A) (n = 3). Inhibition of the p50 and p65 subunits individually had no effect on IL-1β-induced cyclooxygenase-2 (COX-2) mRNA expression. (B) Combined inhibition of both subunits abrogated this effect (representative gel).
