Skip to main content
. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2011 May 6;10(7):1137–1148. doi: 10.1158/1535-7163.MCT-10-1028

Figure 4.

Figure 4

Relevance of active Akt and induced genes to PIA-induced cytotoxicity. A) FACS analysis of sub-G1 DNA content and immunoblotting analysis of active Akt and cleaved PARP in MyrAkt1-expressing H157 cells treated with PIA23 (18h). B) Validation of Akt isoform specific knockdown by shAkt1, 2 or 3 in A549 cells and effect of individual stable knockdown on expression level of total and active Akt, as well as PIA23-induced apoptosis (24h). C) Expression of RhoB, KLF6 and p21 in MyrAkt1 or vector-expressing H157 cells treated with PIA23 (18h). D) Expression of RhoB, KLF6 and p21 in shAkt1 or non-targeting vector transfected A549 cells treated with PIA23 (24h). E) Expression of RhoB, KLF6 and p21 in H157 cells pretreated with LY294002 (0.5h), followed by treatment with PIA23 (6h). F) Effect of transient RhoB, KLF6, p21 or non-targeting (NT) siRNA transfection (48h) on protein induction by PIA23 (6h) and PIA-induced sub-G1 content in H157 cells (12h). G) Effect of overexpression of RhoB, KLF6 and p21 or the combination of all 3 genes on viability in H157 cells (48h). PIA23 and LY294002 = 10 μM. Data are means ± s.d. of triplicates and representative of 3 independent experiments.