Table 1.

Cytokine measurements in media (pg/mL ± SEM) of macrophage and trophoblast cultures. The trophoblast, macrophage, and co-culture data are the same data presented in Figure 2. To investigate the synergistic effect of the two cell types in coculture, the p value was determined by comparing the sum of individually cultured trophoblasts plus individually cultured macrophages (column labeled Sum of Individual Troph and Macro) to macrophages co-cultured with trophoblasts (column labeled Coculture Macro-Troph).

Cytokine	Trophoblast	Macrophage	Sum of Individual Troph and Macro	Co-culture Macro-Troph	p-value
Decidual Macrophage Experiments
IL-6	1740.2 ± 331.8	1983.5 ± 472.2	3723.7	6268.2 ± 1136.2	0.0005
IL-18	5.7 ± 1.5	17.2 ± 3.5	22.9	26.8 ± 3.5	0.0327
IL-10	0	55.7 ± 18.2	55.7	107.4 ± 30.9	0.0215
CSF2	53.1 ± 13.9	911.1 ± 377.2	964.2	440.2 ± 154.6	0.0002
TNF	53.7 ± 20.2	562.4 ± 207.9	616.1	303.0 ± 98.2	0.002
CXCL8	7803.5 ± 1013.3	47806.0 ± 14954.3	55609.5	44374.0 ± 11694.2	0.0034
CCL3	268.2 ± 69.6	6825.0 ± 1788.2	7093.2	4469.5 ± 1289.1	0.0002
CCL4	7.8 ± 3.8	151.6 ± 38.9	159.4	120.4 ± 35.9	0.0002
VEGF	51.1 ± 10.6	252.2 ± 33.3	303.3	225.8 ± 43.4	0.0134
CCL2	1962.8 ± 409.5	31210.0 ± 10696.1	33172.8	21711.2 ± 5951.4	0.0681
IL-1ra	301.6 ± 99.0	305.4 ± 97	607	733.1 ± 155.6	0.3125
CSF3	248.7 ± 106.4	1740.2 ± 476.9	1988.9	1522.8 ± 401.9	0.0674
Peripheral Blood Derived Macrophage Experiments
CSF2	62.5 ± 9.6	5.6 ± 3.7	68.1	34.6 ± 9.9	0.0313
VEGF	74.2 ± 16.3	14.6 ± 6.7	88.8	42.3 ± 13.2	0.0313