Figure 2.
KLF4 overexpresssion inhibits growth of 4T1 cells. (A) Representative density plots from FACS analysis of 4T1 cells infected with either AdGFP or AdKLF4 for 72 hours. Cells in the upper right quadrant are positive for BrdU incorporation. Cells in the bottom left quadrant represent cells in the sub-G1 phase. (B) Quantitation of BrdU incorporation (*P < .02) and (C) sub-G1 population (P = .08). (D) Representative Western blot showing increased levels of cleaved caspase 3 and cleaved caspase 7 in AdKLF4-4T1 cells compared with AdGFP-4T1 control cells. Experiments were performed at least three times in triplicate.