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. 2011 Jul;13(7):601–610. doi: 10.1593/neo.11260

Figure 3.

Figure 3

KLF4 inhibits primary tumor growth of 4T1 cells. (A) Western blot shows maintained (in vitro) expression of virally transduced KLF4 in 4T1 cells at days 4 and 7 after infection. (B) 4T1 (n = 5 per group) and (C) 4T1-luc (n = 4 per group) tumors expressing AdKLF4 have reduced volume compared with AdGFP control tumors. Tumors were measured using calipers at various intervals during a 2-week period. (D) AdKLF4 significantly reduces primary tumor formation determined by whole animal bioluminescence scanning at day 5 after injection (AdGFP, n = 9; AdKLF4, n = 10). Luminescence is expressed as photons/second per region of interest. (E) Bioluminescent images of three representative animals from each group in D. The relationship between color and light intensity in arbitrary units is given by the color bar to the right of each group. (F) Immunohistochemical staining of primary AdGFP-4T1 and AdKLF4-4T1 mammary tumors dissected at day 5 after injection, using anti-flag to detect exogenous KLF4. *P < .01. **P < .005. ***P < 5.0e - 6.