Figure 2.

Extracellular ACCA does not inhibit O₂ consumption rate of U87-MG glioma cells. (A) Upper trace: Cells (0.5 x 10⁶) were added to DMEM/F12 medium maintained at room temperature (arrow 1) and rates of O₂ consumption traced for 10 minutes; ACCA was added to 10 mM final (arrow 2) and respiration measured for another 10 minutes, followed by addition of digitonin (40 µM final). Lower trace: O₂ consumption of cells incubated for a similar duration but in the absence of ACCA or digitonin. Lines A, B, and C mark the time intervals that were used to plot O₂ consumption rates (n = 3) at each step (B).