Table 1.
MIC and MBC determination by different methodsa
| Antibiotics | Microdilution method/literature data (MIC) μg/mL |
Our data (MIC) μg/mL |
Microdilution method/literature data (MBC) μg/mL |
Our data (MBC) μg/mL |
||||
|---|---|---|---|---|---|---|---|---|
| Microdilution method | Direct cell counting | BacLight™ staining | Microdilution method | Direct cell counting | BacLight™ staining | |||
| Doxycycline | 0.06–2.00 | 0.4 | >25 | >25 | 0.25–6.40 | 25 | >200 | >200 |
| Tigecycline | 0.006 | 0.015 | >5 | >5 | 0.05 | 0.125 | >10 | >10 |
| Amoxicillin | 0.03–2.00 | 0.3 | >100 | >100 | <0.03–32.00 | 5 | >200 | >200 |
| Metronidazole | 0.06–32.00 | 0.3 | >250 | >250 | >4 | 10 | >500 | >500 |
| Tinidazole | – | 0.09 | >62.5 | >62.5 | >128 | 10 | >250 | >250 |
Notes:
a
Comparison of MIC and MBC values for different antibiotics by standard microdilution method (published literature and our data) and novel direct cell counting and fluorescent BacLight™ staining methods in reference to spirochete forms of Borrelia burgdorferi B31.
Abbreviations: MBC, minimum bactericidal concentration; MIC, minimum inhibitory concentration.