Locomotor effects of intra-VTA PTX on post-treatment day 18–19. Adult, virgin female rats were infused bilaterally into the VTA with PTX (0.1 or 0.3 μg/hemisphere) or its inactive control. Locomotor activity was monitored over 10 hours (dark phase) on post-treatment day 18–19. Data are mean photobeam breaks (± SEM) for groups of 9–11 animals. Panel A (1 hour intervals): Two-Way repeated measures ANOVA Group [F(2, 243)=12.99, P<0.001], Time [F(9, 243)=4.4, P<0.001], Group X Time [F(18, 243)=1.812, P<0.01]. Panel B (collapsed across 10 hours): One-Way ANOVA – Group [F(2, 27)=12.32, P<0.001]. SNK: *P<0.05 vs. all groups.