Locomotor effects of intra-VTA PTX on post-treatment day 6–7. Adult, virgin female rats were infused bilaterally into the VTA with PTX (0.3 μg/hemisphere) or its inactive control. Locomotor activity was monitored over 10 hours (dark phase) on post-treatment day 6, a time-frame corresponding with the emergence of stimulated maternal responding. Data are mean photobeam breaks (± SEM) for groups of 5–7 animals. Panel A (1 hour intervals): Two-Way repeated measures ANOVA Group [F(1, 90)=1.257, P<0.288], Time [F(9, 90)=3.56, P<0.001], Group X Time [F(9, 90)=1.53, P<0.150]. Panel B (collapsed across 10 hours): Student's t-test [T(10)=1.12, P<0.288].