Table 1.
Hemoglobin Beta-Chain Is Modified at Specific Cysteine Residues
| Peptide | MH+ | z | P (pro) P (pep) | Score XC | Coverage DeltaCn |
|---|---|---|---|---|---|
| (A) Untreated Hb | |||||
| Alpha globin; alpha-2 globin (Homo sapiens) | 7.99E-13 | 70.30 | |||
| K.VADALTNAVAHVDDMPNALSALSDLHAHK.L | 2,996.48944 | 3 | 1.53E-05 | 5.30 | 0.55 |
| K.TYFPHFDLSHGSAQVK.G | 1,833.89186 | 2 | 2.93E-07 | 4.46 | 0.61 |
| K.VGAHAGEYGAEALER.M | 1,529.73430 | 2 | 1.08E-11 | 4.29 | 0.60 |
| K.FLASVSTVLTSK.Y | 1,252.71473 | 2 | 2.06E-04 | 3.60 | 0.53 |
| K.KVADALTNAVAHVDDMPNALSALSDLHAHK.L | 3,124.58441 | 3 | 7.99E-13 | 6.06 | 0.55 |
| K.LRVDPVNFK.L | 1,087.62586 | 2 | 2.43E-04 | 2.46 | 0.25 |
| K.LLSHCLLVTLAAHLPAEFTPAVHASLDK.F | 2,967.61246 | 2 | 7.46E-04 | 4.41 | 0.69 |
| K.TYFPHFDLSHGSAQVK.G | 1,833.89186 | 3 | 1.36E-06 | 2.96 | 0.58 |
| K.LRVDPVNFK.L | 1,087.62586 | 2 | 1.45E-03 | 2.49 | 0.41 |
| Beta globin; Hb beta-chain (H. sapiens) | 2.96E-12 | 100.27 | |||
| K.SAVTALWGK.V | 932.51999 | 1 | 8.78E-03 | 1.66 | 0.18 |
| R.FFESFGDLSTPDAVMGNPK.V | 2,058.94772 | 2 | 6.95E-05 | 4.12 | 0.47 |
| K.VLGAFSDGLAHLDNLK.G | 1,669.89080 | 2 | 2.96E-12 | 5.24 | 0.43 |
| K.VVAGVANALAHK.Y | 1,149.67387 | 2 | 1.07E-07 | 3.82 | 0.46 |
| K.VNVDEVGGEALGR.L | 1,314.66482 | 2 | 9.37E-05 | 4.34 | 0.51 |
| K.EFTPPVQAAYQK.V | 1,378.70014 | 2 | 2.27E-03 | 2.56 | 0.26 |
| R.LLGNVLVCVLAHHFGK.E | 1,719.97269 | 2 | 3.12E-03 | 2.40 | 0.26 |
| K.LHVDPENFR.L | 1,126.56398 | 2 | 1.72E-04 | 2.38 | 0.33 |
| K.VNVDEVGGEALGR.L | 1,314.66482 | 1 | 9.71E-08 | 3.32 | 0.54 |
| K.SAVTALWGK.V | 932.51999 | 2 | 2.91E-05 | 2.21 | 0.50 |
| K.KVLGAFSDGLAHLDNLK.G | 1,797.98576 | 2 | 3.96E-11 | 5.31 | 0.58 |
| K.GTFATLSELHCDK.L | 1,421.67294 | 2 | 2.02E-06 | 2.84 | 0.40 |
| (B) Diallyl disulfide-treated Hb | |||||
| Alpha globin; alpha-2 globin (H. sapiens) | 1.42E-11 | 40.27 | |||
| K.KVADALTNAVAHVDDMPNALSALSDLHAHK.L | 3,124.58441 | 2 | 3.34E-06 | 3.67 | 0.52 |
| K.TYFPHFDLSHGSAQVK.G | 1,833.89186 | 2 | 4.69E-07 | 4.47 | 0.62 |
| K.VADALTNAVAHVDDMPNALSALSDLHAHK.L | 2,996.48944 | 2 | 5.67E-06 | 5.48 | 0.64 |
| K.VGAHAGEYGAEALER.M | 1,529.73430 | 2 | 1.42E-11 | 3.81 | 0.55 |
| Beta globin; Hb beta-chain (H. sapiens) | 1.14E-08 | 90.25 | |||
| K.EFTPPVQAAYQK.V | 1,378.70014 | 2 | 1.14E-08 | 2.61 | 0.44 |
| K.GTFATLSELHC*DK.L | 1,493.67294 | 2 | 1.55E-05 | 3.44 | 0.52 |
| K.KVLGAFSDGLAHLDNLK.G | 1,797.98576 | 2 | 3.19E-06 | 4.60 | 0.49 |
| K.LHVDPENFR.L | 1,126.56398 | 2 | 6.72E-04 | 2.55 | 0.32 |
| R.LLGNVLVC*VLAHHFGK.E | 1,791.97269 | 2 | 9.17E-03 | 2.77 | 0.40 |
| K.SAVTALWGK.V | 932.51999 | 2 | 8.84E-05 | 2.38 | 0.41 |
| K.VLGAFSDGLAHLDNLK.G | 1,669.89080 | 2 | 1.39E-08 | 4.91 | 0.51 |
| K.VVAGVANALAHK.Y | 1,149.67387 | 2 | 2.33E-05 | 3.63 | 0.51 |
The purified HB protein from either (A) control or (B) DADS-treated samples was digested with trypsin and subjected to tandem mass spectrometry analysis. Peptides identified from the alpha- and beta-chains of Hb are shown. The modified cysteine residues on the beta-chain from the DADS-treated samples are indicated in bold in B.