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BMP-induced osteogenic and adipogenic differentiation in MSCs. (A) Osteogenic differentiation. C3H10T1/2 cells were infected with AdBMPs or AdGFP for 7 days, followed by histochemical staining of early osteogenic marker ALP activity. (B) Adipogenic differentiation. C3H10T1/2 cells were infected with AdBMPs or AdGFP for 20 days, followed by Oil Red-O staining. PPARγ agonist Troglitazone (TRO, 10 μM) was used as a positive control.
