Skip to main content
. 2008 Jul 10;18(4):545–558. doi: 10.1089/scd.2008.0130

FIG. 3.

FIG. 3.

BMP-induced osteogenic and adipogenic differentiation in MSCs is mutually exclusive. (A) ALP and Oil Red-O costaining. C3H10T1/2 cells were infected with AdBMP-2, −4, −6, −7, −9, or AdGFP. At Day 14, cells were fixed and first stained for ALP activity, followed by Oil Red-O staining. (B) Transmission electron microscope analysis of BMP-induced MSC osteogenic and adipogenic differentiation. C3H10T1/2 cells were infected with the indicated AdBMP-2, AdBMP-9, or AdGFP. At Day 7 and Day 14, cells were processed for transmission electron microscopy (TEM) analysis. Magnification, 12,000×. Abbreviations: AP, membrane-bound ALP activity; LP, accumulated lipid droplets.