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. 2008 Jul 10;18(4):545–558. doi: 10.1089/scd.2008.0130

FIG. 5.

FIG. 5.

Effect of overexpression of Runx2 or PPARγ2 on BMP-induced osteogenic and adipogenic differentiation. (A) Synergistic effect of Runx2 on BMP-induced ALP activity. C3H10T1/2 cells were coinfected with BMP-2, −6, −9, GFP, and/or Runx2 adenoviral vectors. ALP activity was assayed at Day 7. (B) Runx2 exhibits no significant effect on BMP-induced adipogenic differentiation. C3H10T1/2 cells were coinfected with BMP-2, −6, −9, GFP, and/or Runx2 adenoviral vectors. Oil Red-O stain was carried out at Day 14. (C) PPARγ2 enhances BMP-induced ALP activity. C3H10T1/2 cells were coinfected with BMP-2, −6, −9, GFP, and/or PPARγ2 adenoviral vectors. ALP activity was analyzed at Day 7. (D) PPARγ2 augments BMP-induced adipogenic differentiation. C3H10T1/2 cells were coinfected with BMP-2, −6, −9, GFP, and/or PPARγ2 adenoviral vectors. Oil Red-O stain was conducted at Day 14. (E) PPARγ2 overexpression enhances both adipogenic and osteogenic differentiation in vivo. C3H10T1/2 cells were transduced with BMP-9, GFP, and/or PPARγ2 adenoviral vectors and implanted subcutaneously (5 × 106 cells per injection) into the flanks of athymic nude mice. After 5 weeks, animals were sacrificed and subjected to microCT analysis (see Fig. 7B) and histologic evaluation. Abbreviations: Ac, adipocyte; MB, mineralized bone matrix.