Table 7.
Assay system | Evidence of inactivation |
---|---|
In vitro | |
Limiting dilution assay | >5.4 log10 reduction of viable T cells |
DNA modification | Approximately one amotosalen adduct per 83 base pairs |
Polymerase chain reaction | Amplification inhibited by amotosalen – DNA adducts |
Cytokine synthesis | Elimination of IL-8, IL-1β synthesis during storage |
In vivo | |
Murine transfusion model | Prevention of TA-GVHD in a murine parent to F1 transfusion model |