Fig. 4.

Description of the S-303 treatment process for RBCs.

The RBC unit is transferred to the mixing container of the processing set which contains an aqueous treatment solution. The GSH and S-303 are reconstituted and then sterilely transferred to the mixing container of the processing set resulting in a final concentration of 0.2 mmol/1 S-303 and 20 mmol/1 GSH. Following addition and mixing with GSH and S-303, the treated RBC unit is transferred to the Incubation container. The RBC unit is incubated at room temperature (20–25 °C) for up to 18 h. At the end of the incubation period the RBC unit is centrifuged, the supernatant expressed and the RBC unit transferred to a storage container with SAG-M.