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Location of the phosphorylation site. The DokA fragment HK (kinase domain) was purified from radioactively labeled and osmotically shocked Dictyostelium cells as described above. The HK fragment is strongly phosphorylated during osmotic shock (t = 20 min, 40 min), whereas no phosphorylation was observed before the shock (t = 0 min). It was shown by immunostaining with the mAb 426/HK antibody that equal amounts of protein were purified at each point in time.
