Fig. 2.

Sequence and ORF map of the composite SePI and comparison with sequenced SaPIs. (A) The 20.5-kb mobile genetic element is inserted into the tmRNA region of S. epidermidis FRI909, between the SsrA-binding protein (GSEF_0621) and a Na⁺-transporting ATPase (GSEF_0143). This island appears to be the product of two independent insertional events, one resulting in the insertion of SePI-1 (flanked by 14-bp attL and attR direct-repeat sequences) and the other in a transposon-mediated insertion. The second insertion, SeCI-1, is flanked by 73-bp direct-repeat sequences and comprises several S. aureus proteins not found in sequenced S. epidermidis genomes. A list of ORFs and predicted functions is presented in Table 1. (B) For comparison to the composite SePI, ORFs in SaPImw2, SaPIn315, and SaPIbov1, which have been functionally characterized (31, 32), are shown.