Fig. 4.

Sequence and ORF analysis of the temperate bacteriophage φ909 in S. epidermidis FRI909. (A) The φ909 bacteriophage insertion is found immediately downstream of the FemC/glutamine synthetase gene (SERP0876 in RP62a; GSEF_0257 in FRI909), a site distinct from the composite genomic island containing SePI-1. The genome shows conserved regions corresponding to those seen in S. aureus phages. (B) Ultrastructure of the bacteriophage showing intact phage particles. Transmission electron microscopy reveals isometric heads (diameter, 60 nm) with long, banded tails and end plates. Arrows mark end plates on tails. (C) PCR amplification of toxin genes from phage DNA and bacterial genomic DNA (gDNA). Lanes 1 and 4, gyrA; lanes 2 and 5, the SEC3 gene; lanes 3 and 6, porF; lane M, marker.