Fig. 3.

Suppression of long filaments in the ΔclpX ΔminC and ΔclpP ΔminC strains. (A) ΔclpX ΔminC (JC0292) cells containing pClpX, pClpX(E185Q), or control vector pBAD24 were grown at 30°C to an OD₆₀₀ of ∼0.4 in the presence of arabinose (0.05%) and ampicillin (100 μg ml⁻¹) and then visualized by DIC microscopy. Bars, 10 μm. (B) Extracts of wild-type (MG1655) or ΔclpX ΔminC cells expressing ClpX or ClpX(E185Q) were immunoblotted with antibodies to ClpX. (C) ΔclpP ΔminC (JC0302) cells containing pClpP or pBAD24 were grown and visualized as described above except the medium contained 0.1% arabinose. Bars, 10 μm. (D) Extracts of wild-type (MG1655) or ΔclpP ΔminC cells expressing ClpP were immunoblotted with antibodies to ClpP.