Table 1.
E. coli strains and plasmids used in this study
| Strain or plasmid | Genotypea | Source, reference, or constructionb |
|---|---|---|
| E. coli strains | ||
| BW25113 | Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ−rph-1 Δ(rhaD-rhaB)568 hsdR514 | 5 |
| JW1165 | BW25113 ΔminC765::kan | 5 |
| JW0428 | BW25113 ΔclpX724::kan | 5 |
| JW0427 | BW25113 ΔclpP723::kan | 5 |
| MG1655 | λ−rph-1 | 10 |
| JC0232 | MG1655 ΔminC::frt | P1(JW1165) × MG1655; pCP20 |
| JC0259 | MG1655 ΔclpX::kan | P1(JW0428) × MG1655 |
| JC0263 | MG1655 ΔclpP::kan | P1(JW0427) × MG1655 |
| JC0292 | MG1655 ΔminC::frt ΔclpX::frt | P1(JW0428) × JC0232; pCP20 |
| JC0302 | MG1655 ΔminC::frt ΔclpP::kan | P1(JW0427) × JC0232 |
| MCZ84 | [araD139] leu-260::Tn10 ftsZ84(Ts) Δ(argF-lac)169 λ−e14−flhD5301 Δ(fruK-yeiR)725(fruA) relA1 rpsL150(Strr) rbsR22 Δ(fimB-fimE)632(::IS1) deoC1 | 15 |
| JC0303 | MCZ84 ΔclpX::kan | P1(JW0428) × MCZ84 |
| JC0304 | MCZ84 ΔclpP::kan | P1(JW0427) × MCZ84 |
| Plasmids | ||
| pCP20 | amp flp recombinase | 13 |
| pBAD24 | amp (expression vector) | 21 |
| pClpX | amp Para::clpX | This study |
| pClpX(E185Q) | amp Para::clpX(E185Q) | This study |
| pClpP | amp Para::clpP | This study |
| pGfp-FtsZ | amp Para::gfp-ftsZ | This study |
| pClpXP | amp PclpP::clpPX | 11 |
a
The kan cassette is flanked by frt sites for removal by FLP recombinase. An frt scar remains after removal of the cassette by using FLP expressed from pCP20.
b
Strain constructions by P1 transduction are described as the P1(donor) × the recipient. Where indicated, removal of the Kan cassette (by use of pCP20) is noted.