Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Description (genotype or relevant features) | Reference(s) or source |
|---|---|---|
| B. cenocepacia strains | ||
| 715j | CF isolate, prototroph | 9,11,25 |
| 715j-ssuR::Tp | 715j with dfrB2 gene inserted in ssuR | 18 |
| 715j-cysB::Tp | 715j with dfrB2 gene inserted in cysB | 18 |
| Plasmids | ||
| pGEM-T Easy | Vector for direct cloning of PCR fragments; Apr | Promega |
| pKAGd4 | Broad-host-range lacZ transcription fusion vector; Apr Cmr | 1,27 |
| pMH611 | Contains whole intergenic region preceding the ssuD ORF (ssuDP, amplified with primers SSUDfw1 and SSUDrev2) in pKAGd4 | 18 |
| pMH639 | Contains ORF cysBBc with His6 codons at the 3′ terminus in the ptrc99A vector under promoter trcP | This study |
| pMH654 | Contains ORF ssuRBc with His6 codons at the 3′ terminus in the ptrc99A vector under promoter trcP | This study |
| pMH689 | tauD1 promoter region (amplified with primers TAUD1fw1 and TAUD1rev2) in the pKAGd4 BamHI site | This study |
| pMH802 | tauD2b promoter region (amplified with primers TAUD2Bfw1 and TAUDBrev2) in the pKAGd4 HindIII-BamHI sites | This study |
| pMH803 | bcal1736 promoter region amplified with primers BCAL1736fw1 and BCAL1736rev2 in the pKAGd4 HindIII-BamHI sites | This study |
| pMH804 | ssuDP2 fragment (amplified with primers SSUDfw21 and SSUDrev22) inserted in the pKAGd4 HindIII-BamHI sites | This study |
| pMH805 | ssuDP1 fragment (amplified with primers SSUDfw25 and SSUDrev2) inserted in the pKAGd4 HindIII-BamHI sites | This study |
| pRI32 | tauD2a promoter region (amplified with primers TAUD2Afw1 and TAUDArev2) in the pKAGd4 BamHI site | This study |
| pRI33 | tauD3 promoter region (amplified with primers TAUD3fw1 and TAUD3rev2) in the pKAGd4 BamHI site | This study |
| pRI34 | tauA promoter region (amplified with primers TAUAfw1 and TAUArev2) in the pKAGd4 BamHI site | This study |
| pRI35 | atsA promoter region (amplified with primers ATSAfw1 and ATSArev2) in the pKAGd4 BamHI site | This study |
| pRI45 | atsAP1 fragment (amplified with primers ATSAfw16 and ATSArev17) in the pKAGd4 HindIII-BamHI sites | This study |