Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
NtcA binds to the sigE upstream intergenic region. Binding of purified 6× His-tagged NtcA to a 207-bp fragment from the intergenic region upstream of sigE was assayed by electrophoretic mobility shift assay (EMSA). Sample mixtures contained 0.1 fmol of labeled DNA fragment with or without 0.6 mM 2-oxoglutarate (2-OG). The last lane contains a 100-fold molar excess of the unlabeled probe as a competitor.
