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Northern RNA blot analysis of nifH transcripts from the wild-type strain and sigE mutant strain AMC649 during heterocyst development. Lanes are labeled with hours after nitrogen step-down from BG-11 to BG-110 medium. The upper panel shows a Northern RNA blot probed with a radioactive probe for the nifH transcript. The lower panel shows an ethidium bromide-stained gel to show sample loading. Three transcripts containing nifH were detected at approximately 1.1 kb, 2.8 kb, and 4.7 kb.
