Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Anabaena strains | ||
| PCC 7120 | Wild-type Anabaena sp. strain PCC 7120 | R. Haselkorn |
| AMC649 | PCC 7120 sigE::luxAB-Smr/Spr cassette at ClaI site of sigE; Smr Spr | 27 |
| AMC1452 | PCC 7120 carrying PsigE-gfpmut2 on pAM3652; Kmr Nmr | 3 |
| AMC1773 | PCC 7120 carrying PpetE-sigE on pAM3961; Kmr Nmr | This study |
| AMC1774 | PCC 7120 with integrated pRL277-PnifHD-gfpmut2; Smr Spr | This study |
| AMC1775 | PCC 7120 with integrated pRL277-PnifHD-gfpmut2 and PpetE-sigE on pAM3961; Smr Spr Kmr Nmr | This study |
| AMC1776 | PCC 7120 with integrated pRL278-PnifHD-gfpmut2; Kmr Nmr | This study |
| AMC1777 | AMC649 carrying pRL278-PnifHD-gfpmut2; Kmr Nmr | This study |
| AMC1789 | PCC 7120 carrying PsigE-P1-gfpmut2 on pAM4469; Kmr Nmr | This study |
| AMC1790 | PCC 7120 carrying PsigE-P2-gfpmut2 on pAM4468; Kmr Nmr | This study |
| AMC1791 | PCC 7120 carrying PsigE-P3-gfpmut2 on pAM4467; Kmr Nmr | This study |
| AMC1792 | PCC 7120 carrying PsigE-P4-gfpmut2 on pAM4466; Kmr Nmr | This study |
| Plasmids | ||
| pAM1956 | Shuttle vector pAM505 containing promoterless gfpmut2 with unique upstream cloning sites SalI, SacI, KpnI, ACC651, and SmaI; Kmr Nmr | 56 |
| pAM2770 | Shuttle vector containing XhoI-PpetE-NdeI-lacZα-SapI(Cys)-6His (stop)-ClaI; Kmr Nmr | 31 |
| pAM3652 | pAM1956 containing 800-bp fragment upstream of sigE (alr4249), PsigE-gfpmut2 | 3 |
| pAM3929 | ntcA cloned into expression plasmid pET-28b+ in EcoRI and XhoI sites | This study |
| pAM3961 | sigE (ORF alr4249) cloned into pAM2770 containing PpetE in NdeI and XmaI sites; Kmr Nmr | This study |
| pAM4466 | pAM1956 containing 79-bp fragment upstream of sigE (alr4249), PsigE-P4-gfpmut2; Kmr Nmr | This study |
| pAM4467 | pAM1956 containing 260-bp fragment upstream of sigE (alr4249), PsigE-P3-gfpmut2; Kmr Nmr | This study |
| pAM4468 | pAM1956 containing 360-bp fragment upstream of sigE (alr4249), PsigE-P2-gfpmut2; Kmr Nmr | This study |
| pAM4469 | pAM1956 containing 500-bp fragment upstream of sigE (alr4249), PsigE-P1-gfpmut2; Kmr Nmr | This study |
| pRL277 | Conjugal suicide plasmid; Smr Spr | 13 |
| pRL278 | Conjugal suicide plasmid; Kmr Nmr | 13 |