Table 8.
Edman N-terminal sequencing data of proteins derived from the ultracentrifuged pellet
| Peptide no.a (band mass [kDa]) | NH2-terminal sequenceb |
Accession no. | |
|---|---|---|---|
| Obtained sequence | Predicted sequence, protein (kDa) | ||
| 1 (167) | MQN{S}QEM{A}I- | MQNSQEMAI, YenB (167) | ABG33866 |
| 2 (156) | {S}NSIEQKLQ | SNSIEAKLQ, YenA2 (156) | ABG33868 |
| 4 (107) | -N{Q}FL(F/S)ALT- | MNQFDSALH, YenC1 (107) | ABG33865 |
| 5 (88) | SNSIEAKLQ | SNSIEAKLQ, YenA2 (156) | ABG33868 |
| 9 (57) | AAKDVKFGN | GroEL (57) YE0354, Yersinia enterocolitica subsp. enterocolitica 8081 | YP001004730 |
| 10a (52) | -{Q}PLTF(E/L)PVV | QPLTFEPVV, YenA2 (156) | ABG33868 |
| 10b (51) | TFEPVVHDQ | TFEPVVHDQ, YenA2 (156) | ABG33868 |
| 10c (50) | DQTMSSAVDN | DQTMSAVDN, YenA2 (156) | ABG33868 |
| 11 (35) | APKDNTWYTc | OmpA (38) signal sequence cleaved is 36 kDa; YE1581; Y. enterocolitica subsp. enterocolitica 8081 (357) | Y_P001005874 |
| 12 (32) | AEIYNKDGNc | OmpC (39.6) signal sequence cleaved is 37.6 kDa; KPK_1516; Klebsiella pneumoniae (342) | Y_P002237369 |
a
See Fig. 4C for band number.
b
Braces around a letter indicate that it was a tentative call. -, no call determined due to cycle interference.
c
N-terminal sequence at the predicted signal sequence cleavage site of its protein orthologue.