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Chitinase assay
| Chitinase | Substrate | Activity (μmol of p-nitrophenol released after 30 min at 37°C) |
||
|---|---|---|---|---|
| TC (0.1 μg/ml) | Standard chitinasea (0.05 μg/ml) | ΔTC strain | ||
| Exochitinase | 4-Nitrophenyl-N-acetyl-β-d-glucosaminide | 0.017 | 0.57 | 0.003 |
| 4-Nitrophenyl-N-N′-diacetyl-β-d-chitobioside | 0.0747 | 0.145 | 0.009 | |
| Endochitinase | 4-Nitrophenyl-N-acetyl-β-d-N,N′,N″-triacetylchitotriose | 0.1211 | 0.108 | 0.005 |
Chitinase from Trichoderma viride (catalog no. CS0980; Sigma Aldrich).
