Table 2.
Primers used in this studya
| Primer | Sequence (5′→3′) | Purpose |
|---|---|---|
| PcomEF | TTTTTTCCATGGGGGTGTCGTCATTCTTCCTT | Construction of pALEC50 |
| PcomER | TTTTTTCCATGGTATTTCTCCTTTAATCTTCTAT | Construction of pALEC50 |
| PcomXF | AAAAAAACCATGGTCCAAAAATAAGTGACTAAGG | Construction of pALEC52 |
| PcomXR | AAAAAAACCATGGCTATTACGATGACCTCCTTT | Construction of pALEC52 |
| GFPF | AAAAAATCTAGAAAGATATCATGAGTAAAGGAGAAGAACTTTTCACTGG | eGFP |
| GFPR | AAAAAAGCTAGCCTATTTGTATAGTTCATCCATGCCATG | eGFP |
| PcomX2F | TCCAAAAATAAGTGACTAAGG | Promoter comX |
| PcomX2R | CTATTACGATGACCTCCTTT | Promoter comX |
| PcipBF | TTTATATCTCCTTTTTTTGATTATAATTAGTATACTA | Promoter cipB |
| PcipBR | ATGATAAATACCCCTTCCCCATTTTTAGTT | Promoter cipB |
| PcomE2F | TTATATAATCAATTGACAACGGCTGAT | Promoter comE |
| PcomE2R | TATTTCTCCTTTAATCTTCTAT | Promoter comE |
a
Restriction sites are in boldface.