Table 3.
B. pseudomallei and select B. thailandensis genes showing increased transcription in response to HrpBbp overexpression
| Locus tag(s) | p2bp boxa | Description/product(s)b | Log2 relative expressionc |
||
|---|---|---|---|---|---|
| Microarray | qRT-PCR | RNA-Seq | |||
| BPSS1611-BPSS1629 | + | T3SS2bp cluster | 3.5 | NA | 9.4 |
| BPSS1604-BPSS1609 | + | Hypothetical proteins and a two-component regulatory system | 3.3 | NA | 9.3 |
| BPSS1593-BPSS1601 | − | Type IV pilus-encoding cluster | 2.9 | NA | 5.5 |
| BPSS0998-BPSS1004 | + | Polyketide biosynthesis cluster | 1.7 | NA | 4.8 |
| BPSS1614 (BTH_II0758d) | + | Ortholog of R. solanacearum hrpW | Spot absent | 10.6 ± 0.7 (11.5 ± 0.3) | 10.6 |
| BPSS1621 (BTH_II0750d) | + | Ortholog of R. solanacearum hrcU | 3.4 ± 0.05 | 10.5 ± 0.1 (10.6 ± 0.6) | 9.8 |
| BPSS1623 (BTH_II0748d) | + | Ortholog of R. solanacearum hrpJ | 4.4 ± 0.01 | 12.2 ± 0.1 (12.0 ± 0.5) | 11.8 |
| BPSS1592 | + | T3SS2bp secretin | 4.5 ± 0.1 | 8.1 ± 0.8 | 8.2 |
| BPSS1606 (BTH_II0764d) | + | Hypothetical protein | 4.2 ± 0.1 | 13.2 ± 0.2 (12.8 ± 0.5) | 13.1 |
| BPSS1003 | + | Hypothetical protein | 1.3 ± 0.03 | 4.7 ± 1.8 | 4.2 |
| BPSS1004 | + | Malonyl CoA-acyl carrier protein transacylase | 1.5 ± 0.08 | 6.3 ± 1.0 | 8.3 |
| BPSS1323 | + | Hypothetical protein | 3.9 ± 0.2 | 13.4 ± 0.4 | 13.3 |
| BPSS0740 | + | Hypothetical protein | 3.0 ± 0.3 | 9.6 ± 0.2 | 10.5 |
| BPSS0310 | + | Putative SET domain protein | 3.2 ± 0.2 | ND | 2.3 |
| BPSS0312 | + | Autoinducer-binding transcriptional regulator | 2.4 ± 0.4 | 3.2 ± 0.4 | 4.3 |
| BPSS1389 | + | Putative hemagglutinin/hemolysin | 1.9 ± 0.6 | 6.2 ± 0.5 | 6.8 |
| BPSS1384 | − | Putative membrane protein | 4.0 ± 0.1 | 7.6 ± 1.3 | 7.8 |
| BPSS0804 | − | Hypothetical protein | 4.3 ± 0.03 | ND | 10.0 |
| BPSS1322 | − | Transcriptional regulator, AraC family | 4.3 ± 0.01 | ND | 8.6 |
| BPSS0803 | − | Hemolysin III-like integral membrane protein | 3.3 ± 0.04 | ND | 6.8 |
| BPSL0006 | − | Putative soluble lytic transglycosylase | 2.1 ± 0.1 | ND | 2.8 |
| BPSS1409 | − | Hypothetical protein | 2.0 ± 0.2 | ND | 6.4 |
| BPSS0995 | − | AraC family transcriptional regulator | 1.9 ± 0.1 | ND | 5.1 |
| BPSS0996 | − | Rieske family iron-sulfur cluster-binding protein | 2.0 ± 0.3 | ND | 4.4 |
a
+, present; −, absent.
b
The first 4 genes were manually annotated; the descriptions of the remaining genes are based upon data from IMG (http://img.jgi.doe.gov).
c
Log2 ratios (± standard deviations) of gene transcript levels in B. pseudomallei or B. thailandensis strains overexpressing HrpBbp relative to that of the wild type. The first four rows of data represent the average upregulation of all genes in the indicated cluster. NA, not applicable; ND, not done.
d
Locus tag of the orthologous gene in B. thailandensis.