Table 2.
Oligonucleotides used in this study
| Oligonucleotide name | Sequence (5′-3′) | Orientation to bimA | Purpose |
|---|---|---|---|
| pCompE1.2 | CTCGAATTCCATGCGTGCAATAGCTG | Forward | Cloning |
| pCompxho.2 | CTTCTCGAGTGCTTACCATTGCCAGCTCAT | Reverse | Cloning |
| Bim117 | GGTGCCGCCCGGCGGTT | Reverse | Δ13AA |
| Bim118 | AACATCCCGCCCCCGC | Forward | Δ13AA |
| Bim119 | GTTCGCGCCGCCGCCCGGCA | Reverse | ΔPRM1 |
| Bim120 | GGCGGCATTGGCGGCGCAA | Forward | ΔPRM1 |
| Bim123 | GTTCGCATCGGGCGCCAGATT | Reverse | ΔWH21 |
| Bim124 | GCATACCTGCCTGCCGAGCG | Forward | ΔWH21 |
| Bim125 | CGGAGCGGCATGGCCGGCG | Reverse | ΔWH22 |
| Bim126 | CAGCCGCGGTCGACACCAC | Forward | ΔWH22 |
| Bim123 | GTTCGCATCGGGCGCCAGATT | Reverse | ΔWH21+2 |
| Bim126 | CAGCCGCGGTCGACACCAC | Forward | ΔWH21+2 |
| Bim121 | TACGTTCGGGTTGGGCGCGG | Reverse | ΔPDASX |
| Bim122 | CCCAGCCGACCTGCCCCT | Forward | ΔPDASX |
| 121+2PDAST | CGTCGATGCATCAGGCGTCGATGCGTCGGGTACGTTCGGGTTGGGCGCGG | Reverse | 2× PDASX |
| 122+3PDAST | CCCGACGCATCGACGCCTGATGCATCGACGCCTGACGCTTCGACGCCCAGCGACCTGCCCCT | Reverse | 7× PDASX |
| 4PDAST | GGCGCGGGTTGGGCTTGCATGGGCTGCGTAGCTGCGGGCTGCGTAGCTGCGGGCTGCGTAGCTGCGGGCTGCGTAGCTGC | Forward | 7× PDASX |
| BimA pGEX start | GCGCGCGGATCCATGAATCCCCCCGAACCGCCGGGC | Forward | Cloning |
| BimA pGEX stop | GCGCGCGAATTCTTAGCGCGCGGTGTCGGTG | Reverse | Cloning |